[STATE REGULATED SCOPE]

TDLR-ACCREDITED IN-HOUSE LABORATORY

TDLR LICENSE #1050 • ZERO OUT-OF-STATE TRANSIT RISK • IMMEDIATE STAT PROCESSING

Envirogyn operates an independent, in-house analytical microbiology laboratory fully licensed and accredited by the Texas Department of Licensing and Regulation (TDLR LAB #1050). Operating an accredited analytical bench within our Texas City headquarters provides an unassailable tactical advantage over assessment firms that outsource diagnostics to third-party mail-in clearinghouses.

The Thermal Degradation Hazard: Shipping optical spore trap cassettes across state lines via commercial freight introduces severe thermal vulnerability. Texas Gulf Coast ambient temperatures routinely exceed 100°F inside cargo trailers, which desorbs adhesive collection media, compromises spore impaction traces, and delays critical containment decisions by 48 to 72 hours.

Unbroken Chain of Custody: By hand-delivering physical field cassettes directly from the assessment zone to our bench microbiologists, Envirogyn guarantees an airtight, cryptographically verified Chain of Custody (eCoC) under Texas Occupations Code § 1958.055, completely eliminating courier transit loss, sample tampering, or administrative misplacement.

BENCH OPTICS // ACTIVE Envirogyn analytical microbiology laboratory bench

INSTRUMENTATION SPECIFICATIONS

HIGH-RESOLUTION OPTICAL BENCH

OPTICAL MAGNIFICATION	100x • 400x • 1000x (OIL IMMERSION)
CALIBRATION STANDARD	STAGE MICROMETER (1.0 µm PRECISION)
QA/QC PROTOCOL	100% TRANSVERSE PASS + BLIND BENCH RE-READS
TURNAROUND SLA	STANDARD 24-HR // EMERGENCY 3-HR STAT
DELIVERY FORMAT	CRYPTOGRAPHIC PDF CERTIFICATE OF ANALYSIS

[BIOAEROSOL FORENSICS]

OPTICAL MICROSCOPY (ASTM D7391)

VOLUMETRIC SPORE ENUMERATION • 100% TRANSVERSE TRACE ANALYSIS • AIR-O-CELL & ALLERGENCO-D

Our primary diagnostic methodology for airborne fungal quantification is direct optical microscopy conducted in strict compliance with ASTM D7391-20. Unlike culturing methods which introduce heavy species selection bias, optical microscopy enumerates both viable and non-viable spores simultaneously, yielding a definitive representation of true airborne biological load.

BIOAEROSOL OPTICS // VOLUMETRIC CAPTURE

CALIBRATED INTAKE

High-velocity air intake drawn through calibrated high-volume bioaerosol pumps at an exact 15.0 L/min flow rate for a 5-to-10 minute window (75L–150L total air mass), ensuring uniform rectangular trace impaction onto specialized optical media without particle bounce.

STANDARD: ASTM D7391 15.0 L/MIN CALIBRATED

BIOAEROSOL OPTICS // BENCH ENUMERATION

100% TRANSVERSE SCAN

While retail production labs scan only 10% to 20% of the trace and multiply mathematically, Envirogyn analyzes 100% of the transverse trace width at 400x/1000x magnification. This guarantees critical toxic marker species (such as Stachybotrys or Chaetomium) are never missed due to sampling bias.

OPTICS: 400x / 1000x 100% TRACE WIDTH

BIOAEROSOL OPTICS // MATHEMATICAL DERIVATION

SPORES/M³ EXTRAPOLATION

Raw spore tallies are converted to total airborne concentration using the standard ASTM formula: (Raw Count / Trace Fraction) × (1000 / Sample Volume L). Concentrations are benchmarked against outdoor reference controls to statistically prove Condition 1, 2, or 3 ecology.

METRIC: COUNT / M³ IICRC S520 BENCHMARKED

[TAXONOMY INTEL ARCHIVE // FIELD BENCH REFERENCE]

ASTM MOLD SPORE FORENSIC ATLAS & TAXONOMY DOSSIER

Access Envirogyn's proprietary interactive taxonomy library: 22 ASTM D7391 airborne and surface fungal genera complete with high-resolution optical morphology, water activity (Aw) thresholds, mycotoxin toxicological profiles, and clinical respiratory pathology.

STACHYBOTRYS CHARTARUM CHAETOMIUM GLOBOSUM ASPERGILLUS / PENICILLIUM CLADOSPORIUM MEMNONIELLA ALTERNARIA CURVULARIA + 15 MORE GENERA

ACCESS SPORE ATLAS →

[SUBSTRATE DIAGNOSTICS]

DIRECT MICROSCOPIC EXAMINATION (DME)

ASTM D7658 STANDARD • BIO-TAPE IMPACTION • BULK EXCISIVE ANALYSIS

Air sampling captures aerosolized transport, but surface diagnostics confirm root colonization. Through Direct Microscopic Examination (DME), our microbiologists distinguish between superficial settled dust (Condition 2) and active fungal propagation with vegetative hyphae, fruiting structures, and substrate penetration (Condition 3).

SUBSTRATE DIAGNOSTICS // ASTM D7658

SURFACE BIO-TAPE IMPACTION

Standardized flexible microscope slides with medical-grade optically clear adhesive are pressed directly onto suspected building materials—drywall, structural framing, HVAC supply plenums, subflooring, and personal property. Direct examination under 400x/1000x brightfield microscopy reveals intact conidiophores and mycelial growth networks.

DENSITY CLASSIFICATION MATRIX:

1+ (Rare / Settled) • 2+ (Few / Suspicious)
3+ (Moderate Colonization) • 4+ (Heavy / Overgrowth)

APPLICATION: NON-DESTRUCTIVE HYPHAL MORPHOLOGY CONFIRMED

SUBSTRATE DIAGNOSTICS // DESTRUCTIVE SAMPLING

BULK & SUBSTRATE EXCISION

Physical material samples—including saturated gypsum core, fiberglass insulation batt, acoustic ceiling tile, and decayed timber framing—are surgically excised and examined under stereo-dissecting and compound optical microscopes. This confirms depth of vegetative penetration, structural rot, and historical moisture events.

STRUCTURAL CORRELATION:

Substrate Porosity Profiling • Demolition Boundary Scoping
Protocol Design Baseline for TDLR MRC Contractors

APPLICATION: STRUCTURAL PROTOCOLS CROSS-SECTIONAL DEPTH

[FIELD LOGISTICS & SUPPLY]

MOLD TEST DIRECT & DIGITAL eCoC PIPELINE

PROFESSIONAL SAMPLING KITS • CRYPTOGRAPHIC CHAIN OF CUSTODY • CLIENT PORTAL

Through our dedicated sampling product division, Mold Test Direct, Envirogyn equips environmental consultants, mechanical contractors, and proactive facility managers with calibrated collection hardware and direct access to our Texas-accredited analytical pipeline.

LOGISTICS // FIELD SAMPLING HARDWARE

MOLD TEST DIRECT HARDWARE KITS

Field kits complete with calibrated rotameter bio-pumps, Air-O-Cell / Allergenco-D cassettes, sterile bio-tape slides, protective thermal transport cases, and prepaid laboratory submission vouchers for seamless integration into our Texas City testing facility.

TARGET: CONTRACTORS & MANAGERS PREPAID LAB CREDITS INCLUDED

PORTAL // ELECTRONIC CHAIN OF CUSTODY

DIGITAL eCoC CLIENT PIPELINE

Paperless, tamper-evident Chain of Custody portal. Field assessors log samples with barcode scans, volumetric calibration records, and GPS coordinates. Clients track custody status in real time and receive instant, encrypted PDF Certificates of Analysis upon final bench verification.

SECURITY: SHA-256 TIMESTAMPING INSTANT SECURE PDF DISPATCH

ANALYTICAL LABORATORY